Laboratories: Bluemle Life Sciences Building 209/219: 1520 ft2. These laboratories assigned to Prof. Wickstrom contain the space and equipment needed for oligonucleotide and peptide synthesis, purification and analysis. 209: 250 ft2, with Shimadzu UV160 spectrophotometer, NanoDrop ND-1000 microspectrophotometer, NanoDrop ND-3300 microfluorimeter, balances (2), pH meter, dry chemicals, gases, freezer. 209A: 420 ft2, with chemical fume hood, CEM Liberty microwave peptide synthesizer, Protein Technologies PS3 peptide synthesizer, Bio-Rad Opticon fluorescence detection thermocycler, Kodak Image Station 2000R, Shimadzu liquid chromatograph and UV detector, Agilent G1600A capillary electrophorometer with diode array detector, vertical and horizontal gel electrophoresis systems, power supplies, rotating platform, Beckman GS-6 batch centrifuge, Eppendorf refrigerated microcentrifuge, water bath, vertical and horizontal gel electrophoresis systems, power supplies, rotating platform, refrigerator/freezers (3). 209B: 90 ft2, with Nu-Aire Class II laminar flow hood, Fisher convection incubator, Olympus inverted microscope, Seiwa inverted microscope, New Brunswick gyratory water bath. 219A: 420 ft2, with chemical fume hood, Vacuum Atmospheres controlled atmosphere glove box, Applied Biosystems 8909 DNA synthesizer, Varian Cary 3 spectrophotometer with kinetics and Peltier accessories, Waters 600 liquid chromatographs and detectors (3), Chromatotron rotary chromatograph, Büchi Rotovapors (2), Welch vacuum pump, Parr hydrogenator, Eppendorf microcentrifuges (2), balances (2), water bath, vertical gel electrophoresis systems, power supplies, rotating platform, refrigerator, freezer.
Common rooms: These common facilities contain the space and equipment needed for cell culture and analysis. BLSB 204: Beckman J2-21 refrigerated centrifuge, Forma -80°C freezer, Savant Speed-Vacs (3). BLSB 213: preparation room with Bio-Rad Gene Pulser, sonic cell disrupter, Coulter counter, liquid N2 cell stock freezers (2), refrigerator, water bath, rotating platform. BLSB 213A: mammalian tissue culture room with Nu-Aire Class II laminar flow hood, Forma CO2 cell culture incubators (2), Eppendorf high-capacity centrifuge, Olympus CK40 inverted digital fluorescence microscope. BLSB 218: autoclave and dishwasher. BLSB 235: dark room with Kodak X-Omat film developer.
Computers: These computers in Prof. Wickstrom’s laboratory provide the ability to model and display molecules, control instruments, analyze data, and write manuscripts. BLSB 209/219: 3D haptic Boxx Technologies 5570 dual-booting Win XP/Linux workstation with dual quad core 2.66 GHz Xeon X5550 processors (8), 48 GB RAM, 3.5 TB RAID 5 hard drive array, Nvidia Quadro FX5800 4 GB graphics card with Tesla C1060 GPU, Nvidia Quadro FX3800 1 GB graphics card, Mitsubishi WD-65837 DLP 3D 65” display, Viewsonic VX2265WM LCD 3D 22” display, with dual SensAble Omni haptic manipulators; 3D haptic Dell Inspiron 530 4x2.4 GHz, 4 GB RAM, with dual SensAble Omni haptic manipulators; Dell Precision T1500, 4x3.06 GHz i7-880, 8 GB RAM, 1 TB SATA HD, 4A, Win XP Pro 64 bit, Ubuntu Linux; Dell Optiplex 990, 4x3.4 GHz i7-2600, 8 GB RAM, 500 GB SATA HD, 4A, Win 7 Pro 64 bit, Ubuntu Linux; Dell Precision 350 2.4 GHz server; Dell Inspiron 530s 2×2.0 GHz, 2 GB RAM; Dell Optiplex 755 2×2.3 GHz, 3 GB RAM; Dell Optiplex GX620 3.0 GHz; Dell Dimension 4600 3.0 GHz; Dell Optiplex GX280 2.8 GHz; Dell Optiplex GX270 2.8 GHz (4); Dell Optiplex GX240 1.6 GHz; Lenovo ThinkCentre M55; 2x2.13 GHz, ASUS Eee PC 1.6 GHz notebook; Xerox 8400 and Xerox 8570 (2) color solid ink printers; HP laser printers (3).
Offices: These offices in Prof. Wickstrom’s laboratory provide workspace for Prof. Wickstrom, research associates, postdoctoral fellows, graduate students, and technicians. BLSB 219B, 90 ft2, 209, 250 ft2, and 219, ft2.
Major Equipment:These common instruments provide sophisticated ability to analyze oligonucleotides, peptides, and cells. BLSB 215: Photon Technologies QuantaMaster double monochromator fluorimeter. BLSB 409: Biotek FL 600 microplate reader with KC4 software. BLSB 606: Coulter EPICS Elite fluorescence activated cell sorter, Coulter XL-MCL automated analytical flow cytometer, a FACS-Calibur benchtop analyzer, and a FACS-VantageSE high speed cell sorter. BLSB 706: Biacore 3000 surface plasmon resonance flow spectrometer. BLSB 815: ABI SciEx 4800 MALDI TOF/TOF mass spectrometer with microflow HPLC inlet, ABI SciEx 4000Q electrospray triple sector mass spectrometer with Shimadzu HPLC inlet. BLSB 822: Jasco J-810 circular dichroism/fluorescence spectrometer with Peltier thermostat, titrator, and stopped-flow kinetics. BLSB 833: Photon Technologies double monochromator fluorimeter with Peltier temperature control, MicroCal isothermal calorimeter, and Sapidyne Kinexa fluorescence flow spectrometer. BLSB 914: Zeiss LSM 510 Meta laser scanning confocal 4 channel fluorescence microscope. BLSB 1009: Nucleic Acid Facility, with ABI 377 sequencers (5) and an ABI 3700 analyzer for DNA sequence determination, and a Qiagen BioRobot 8000 for rapid, high throughput DNA purification. BLSB 1015: Microarray Facility, with Gene Machines oligonucleotide spotter, Tecan reaction robot, Packard BioScience ScanArray Express fluorescent chip reader, and ABI Prizm 7700 real time PCR fluorescence spectrometer. JeffIT: High-Performance Computing Cluster (HPCC) with 8 Dell Poweredge servers, each with 2 dual core 2.66 GHz processors and 4 GB of RAM, a head node with 2 dual core 2.66 GHz processors, and 8 GB of RAM, and a 2-TB file storage array, running RedHat Linux 9.
Additional Information: BLSB 302/350: Departmental offices, for purchasing, grant management, and document preparation. Jefferson Alumni Hall: electronics shop, machine shop, media services.
Research Environment: TThe Kimmel Cancer Center, the Cardeza Foundation for Hematologic Research, the Farber Institute for Neurosciences, the Daniel Baugh Institute for Functional Genomics, the Jefferson Institute for Molecular Medicine, and the Jefferson Institute for Translational Medicine provide a strong nucleus of investigators and facilities.