Curriculum Vitae
1972-1979: Master of Sciences, Chemistry, Novosibirsk State University, Novosibirsk, Russia
1979-1982: Senior Technician, Department of Genetic Engineering, Institute of Bioorganic Chemistry, Uzbekistan Academy of Sciences, Tashkent, Uzbekistan
1982-1985: Research Engineer, Department of Genetic Engineering, Institute of Cytology and Genetics, Novosibirsk, Russia
1985-1989: Doctor of Philosophy, Institute of Bioorganic Chemistry, Novosibirsk, Russia
1989-1999: Research Scientist, Department of Nucleic Acids Chemistry, Institute of Bioorganic Chemistry, Novosibirsk, Russia
1999-2002: Visiting Researcher, Department of Bioorganic Chemistry, Biomedical Center, Uppsala University, Uppsala, Sweden
2003-present: Research Associate, Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania
Publications
1. Amirkhanov, N. V., Kumarev, V.P., Rivkin, M. I., Rybakov, V. N. Solid-phase synthesis of polydeoxyribonucleotides by a modified phosphotriester method. Bioorganicheskaya Khimiya, 1982, v. 8, No 1, pp. 126-129. Russian.
2. Amirkhanov, N. V., Rivkin, M. I., Kumarev, V. P. Solid phase synthesis of oligo- and polynucleotides. II. Solid phase synthesis of heptadecadeoxyrybonucleotide T-C-A-T-T-C-C-T-T-A-C-T-C-T-T-C-A by phosphotriester method using protected 5'-nucleotides. Bioorganicheskaya Khimiya, 1982, v. 8, No 7, pp. 1008-1010. Russian.
3. Amirkhanov, N. V., Kuznedelov, K. D., Rivkin, M. I., Kumarev, V. P. Cloning of single-stranded synthetic DNA. Bioorg Khim. 1985, v. 11, No 9, pp. 1283-1285. Russian.
4. Kumarev, V. P., Rivkin, M. I., Amirkhanov, N. V., Baranova, L. V., Bogachev, V. S., Kobets, M. L., Oshevski, S. I., Obuhova, L. V., Rybakov, V. N., Kuznedelov, K. D., Vershinina, S. I., Gulevich, V. V. Chemical-enzymatic synthesis and cloning of a biologically active beta-interferon gene. Doklady Biochemistry 1986, v. 290, pp. 296-300. Translated from Dokl Akad Nauk SSSR, 1986, v. 290, No 1, pp. 244-249. Russian.
5. Kumarev, V. P., Rivkin, M. I., Amirkhanov, N. V., Baranova, L. V., Bogachev, V. S., Kobets, M. L., Oshevski, S. I., Obuhova, L. V., Rybakov, V. N., Kuznedelov, K. D., Vershinina, S. I., Gulevich, V. V. Method of the natural beta-interferon gene preparation. Patent RF, No 1362016, C 12 N 15/00, 22.08.1987.
6. Kumarev, V. P., Rivkin, M. I., Amirkhanov, N. V., Baranova, L. V., Bogachev, V. S., Kobets, M. L., Oshevski, S. I., Obuhova, L. V., Rybakov, V. N., Kuznedelov, K. D., Vershinina, S. I., Gulevich, V. V. Method of the preparation of the polypeptide with beta-interferon activity. Patent RF, No 1362017, C 12 N 15/00, 22.08.1987.
7. Amirkhanov, N. V. Method of the deoxynucleotide derivatives preparation. Patent RF, No 1487425, C 07 H 19/20, 15.02.1989.
8. Amirkhanov, N. V., Zarytova, V. F. Octathymidylate alkylating derivatives. Biopolimery i Kletka, 1989, v. 5, No 1, pp.102-105. (Ukrainian).
9. Amirkhanov, N. V., Zarytova, V. F. Reactive derivatives of oligonucleotides containing methylphosphonate groups. I. Synthesis and separation of 5'-O-dimethoxytrityl [thymidylyl-(3'-methylphosphonate-5')-thymidine]-3'-0- -cyanoethyl-S-methylthiophosphate. Soviet J. Bioorganic Chemistry, 1989, v. 15, No 2, pp. 142-148. Translated from Bioorganicheskaya Khimiya, 1989, v. 15, No 2, pp. 259-266. Russian.
10. Amirkhanov, N. V., Zarytova, V. F. Reactive derivatives of oligonucleotides containing methylphosphonate groups. II. Synthesis of stereoregular octathymidylates containing an alkylating 4-(N-2-chloroethyl-N-methylamino)benzylamine group and alternating methylphosphonate residues. Soviet J. Bioorganic Chemistry, 1989, v. 15, No 2, pp. 149-157. Translated from Bioorganicheskaya Khimiya,1989, v. 15, No 2, pp. 267-276. Russian.
11. Amirkhanov, N. V., Zarytova, V. F. Reactive derivatives of oligonucleotides containing methylphosphonate groups. III. Affinity modification of target DNA by 4-N-(methyl-N-2-chlorethylamino)benzyl 3'- and 5'-phosphamide derivatives of octathymidylate containing methylphosphonate residues. Soviet J. Bioorganic Chemistry, 1989, v. 15, No 3, pp. 217-223. Translated from Bioorganicheskaya Khimiya, 1989, v. 15, No 3, pp. 379-386. Russian.
12. Amirkhanov, N. V.: Method of the preparation 3'-phosphate, N, P-unprotected oligonucleotide phosphorothioate analogues. Patent RF, No 2131881, C 07 H 21/00, 21/04, BI No 17, 20.06.1999.
13. Amirkhanov, N. V., Zarytova, V. F. Reactive derivatives of oligonucleotides containing methylphosphonate groups. IV. Stabilization of complementary complexes of methylphosphonate octathumidilates by N-(2-hydroxyethyl)phenazinium residue. Soviet J. Bioorganic Chemistry, 1990, v. 15, No 12, pp. 871-878. Translated from Bioorganicheskaya Khimiya, 1989, v. 15, No 12, pp. 1618-1626.
14. Amirkhanov, N. V., Zarytova, V. F. Reactive derivatives of oligonucleotides containing methylphosphonate groups. V. Increased effectiveness of complementary addressed modification of target DNA by alkylating derivatives of methylphosphonate analogs of octathymidylate containing N-(2-hydroxyethyl)phenazinium residues. Soviet J. Bioorganic Chemistry, 1990, v. 16, No 3, pp. 199-206. Translated from Bioorganicheskaya Khimiya, 1990, v. 16, No 3, pp. 370-377.
15. Amirkhanov, N. V., Zarytova, V. F., Levina, A. S. Reactive derivatives of oligonucleotides containing methylphosphonate groups. VI. Increase in the effectiveness of directed action of alkylating derivatives of oligonucleotide methylphosphonate analogs on nucleic acids in the presence of effectors - 3',5'-bis-N-(2-hydroxyethyl)-phenazine derivatives of oligonucleotides. Soviet J. Bioorganic Chemistry, 1990, v. 16, No 11, pp. 872-879. Translated from Bioorganicheskaya Khimiya, 1990, v. 16, No 11, pp. 1523-1530.
16. Ivanova, E. M., Amirkhanov, N. V., Zarytova, V. F. Steroid derivatives and methylphosphonate analogs of oligodeoxyribonucleotides carrying alkylating groups. Collection of Czechoslovak Chemical Communications, special issue 1 (1990), v. 55, pp. 217-220.
17. Abramova, T. V., Amirkhanov, N. V., Gorn, V. V., Zarytova, V. F., Frolova, E. I., Shishkina, I. G. Affinity chromatography of DNA fragments and oligonucleotides blocked by internucleotide phosphates. Soviet J. Bioorganic Chemistry, 1991, v. 17, No 2, pp. 127-134. Translated from Bioorganicheskaya Khimiya, 1991, v. 17, No 2, pp. 232-240.
18. Ryte, A. S., Amirkhanov, N. V., Koshkin, A. A., Nechaeva, M. B., Ivanova, E. M., Zarytova, V. F., Vlassov, V. V.: Interaction of modified oligonucleotides with eukaryotic and mycoplasma cells and their fate inside the cells. Nucleic Acids Symp Ser 1991, v. 24, p. 285.
19. Panchenko, L. P., Egorov, O. V., Rait, A. S., Ivanova, E. M., Amirkhanov, N. V., Zarytova, V. F., Vlasov, V. V., Skripal', I. G. The interaction of alkylating derivatives of oligodeoxyribonucleotides and their methylphosphonate analogs with Mycoplasma cells. Mikrobiol. Zh. 1991, v. 53, No 4, pp.63-68. Russian.
20. Lokhova, I. A., Nevinskii, G. A., Amirkhanov, N. V., Godovikova, T. S., Ivanova, E. M., Kavsan, V. M., Koshkin, A. A., Levina, A. S., Frolova, E. I., Zarytova, V. F., Lavrik, O. I. The effect of various substituents, joined through the 5'- and 3'-ends of the primer, initiating properties during the polymerization reaction, catalyzed by AMV-revertase. Mol Biol (Mosk), 1993, v. 27 (4), pp.781-789. Russian.
21. Amirkhanov, N. V., Zarytova, V. F. A new approach to the synthesis of the derivatives of phosphorothioate oligonucleotide analogues. Nucleic Acids Symposium Series, 1994, No 31, pp.51-52.
22. Amirkhanov, N. V., Zarytova, V. F. Reactive derivatives of phosphorothioate oligonucleotide analogs. I. Selective activation of terminal phosphates and preparation of phosphamide derivatives of phosphorothioate oligonucleotide analogs. Russian Journal of Bioorganic Chemistry. 1995, v. 21, No 5, pp. 313-322. Translated from Bioorganicheskaya Khimiya, 1995, v. 21, No 5, pp. 365-375.
23. Amirkhanov, N. V., Zarytova, V. F. Synthesis of the 3'-derivatives of phosphorothioate oligonucleotide analogues. Nucleosides & Nicleotides. 1995, v. 14, No 3-5, pp. 935-937.
24. Egorov, O. V., Panchenko, L. P., Skripal', I. G., Amirkhanov, N. V. The binding and absorption by mollicute cells of antisignature analogs of oligodeoxyribonucleotides. Mikrobiol Zh. 1996, 58, No 6, pp.18-29. Russian.
25. Amirkhanov, N. V., Zarytova, V. F. 3'-Phosphate, N, P-unprotected phosphorothioate oligodeoxynucleotides as starting matirials for the preparation of phosphorothioate oligonucleotide reagents applying for biotechnological processes. Patent RF, No 2069214, C 07 H 21/00, BI No 32, 20.11.1996.
26. Amirkhanov, N. V., Zarytova, V. F. The derivatives of phosphorothioate oligonucleotides. Nucleosides & Nicleotides. 1997, vol. 16, No 7-9, pp. 1563-1564.
27. Amirkhanov, N. V., Zarytova, V. F. Reactive derivatives of phosphorothioate oligonucleotide analogues. II. Properties of phosphorothioate oligodeoxyribonucleotide derivatives containing an aromatic analogue of nitrogen lost. Russian Journal of Bioorganic Chemistry. 1997, 23, No 7, pp. 525-530. Translated from Bioorganicheskaya Khimiya, 1997, 23, No 7, pp. 569-575.
28. Amirkhanov, N. V., Neronova, N. V. Irregular temperature dependence of the efficiency of nucleic acids site-directed modification by oligonucleotide reagents producing reactive intermediates. Russian Journal of Bioorganic Chemistry. 1998, 24, No 1, pp. 32-34. Translated from Bioorganicheskaya Khimiya, 24, No 1, 1998, pp. 38-41.
29. Amirkhanov, N. V. Simulation of a temperature dependence of the efficiency of complementary targeted modification of nucleic acids by oligonucleotide reagents producing active intermediates: Detection and Prediction of new specific features of the modification. Doklady Biochemistry, 1998, 359, pp. 31-34. Translated from Doklady Akademii Nauk, 1998, 359, No 3, pp. 405-408.
30. Amirkhanov, N. V., Neronova, N. V., Markushin, Yu. Ya., Yamkovoi, V. I., Zarytova, V.F. Reactive derivatives of phosphorothioate oligonucleotide analogues. III. Synthesis of 3'- and 5'-phosphoroamidate alkylating derivatives of phosphorothioate oligonucleotide analogues containing an aromatic residue of nitrogen lost. Russian Journal of Bioorganic Chemistry. 1999, 25, No 5, pp. 337-345. Translated from Bioorganicheskaya Khimiya, 1999, 25, No 5, pp. 383-392.
31. Laktionov, P. P., Bryksin, A. V., Rykova, E. Iu., Amirkhanov, N. V., Vlasov, V. V. Pharmacokinetics and stability in the blood in vivo of phosphodiester and modified oligonucleotide derivatives. Vopr Med Khim. 1999, v.45, No 3, pp. 206-215. Russian.
32. Amirkhanov, N. V., Neronova, N. V., Zarytova, V. F. Reactive derivatives of oligonucleotide phosphorothioate analogues. IV. Site-directed modification of nucleic acids in intramolecular alkylation of phosphorothioate groups. Russian Journal of Bioorganic Chemistry. 2000, 26, No 2, pp. 125-133. Translated from Bioorganicheskaya Khimiya, 2000, 26, No 2, pp. 141-150.
33. Amirkhanov, N. V., Zarytova, V. F., Plyasunova, O. A., Pokrovski, A. G. The inhibitor of HIV reproduction. Patent RF, No 2144956, C 12 N 7/04, A 61 K 31/70, C 07 H 21/00, C 12 Q 1/68, BI No 3, 27.01.2000.
34. Zamaratski, E., Ossipov, D., Pradeepkumar, P. I., Amirkhanov, N., Chattopadhyaya, J. The 3'-modified antisense oligos promote faster hydrolysis of the target RNA by RNase H than the natural counterpart. Tetrahedron. 2001, 57, No 3, pp. 593-606.
35. Amirkhanov, N.V., Zamaratski, E., Chattopadhyaya, J.: The recognition and cleavage of RNA in the antisense oligo-RNA hybrid duplexes by RNase H. Tetrahedron Letters. 2001, 42, No 3, pp. 489-491.
36. Amirkhanov, N. V., Chattopadhyaya, J. RNase H affinity and cleavage of the target RNA in antisense-RNA hybrid duplexes containing various 3'-tethered substituents in the antisense strand. J. Chem. Soc., Perkin Trans. 2, 2002, (2), 271-281.
37. Amirkhanov, N. V., Pradeepkumar, P. I., Chattopadhyaya, J. Kinetic analysis of the RNA cleavage of the conformationally-constrained oxetane-modified antisense-RNA hybrid duplex by RNase H. J. Chem. Soc., Perkin Trans. 2, 2002 (5), 976-984.
38. Pradeepkumar, P. I., Amirkhanov, N. V., Chattopadhyaya J. Antisense oligonucleotides with oxetane-constrained cytidine exhibit enhanced heteroduplex stability, elicit satisfactory RNase H response, and show improved resistance to both exo and endonucleases. J. Chem. Soc., Perkin Trans. 2, 2003 (Submitted).
Service
Reviewer, Professional Journals