Laboratories: Bluemle Life Sciences Building 209/219: 1520 ft². 209: 420 ft², with MJ Opticon fluorescence detection thermocycler, MJ PTC-100 thermocycler, Kodak Image Station 440CF, Shimadzu UV160 spectrophotometer, vertical and horizontal gel electrophoresis systems, power supplies, rotating platform, Seiwa inverted microscope, Sorvall T21 refrigerated centrifuge, Shandon Cytospin II centrifuge, Eppendorf refrigerated microcentrifuges (2), water bath, vertical and horizontal gel electrophoresis systems, power supplies, rotating platform, refrigerator/freezers (2), freezer; 209A: 90 ft², with Nu-Aire Class II laminar flow hood, Forma CO₂ incubator, Fisher convection incubator, Labline 3526 rotary incubator, Beckman GS-6 batch centrifuge, Olympus inverted microscope, New Brunswick gyratory water bath; 219: 420 ft², with Applied Biosystems DNA synthesizers (8800: 1000 µmol scale; 8909: 35 µmol scale; 8400: one µmol scale), Vacuum Atmospheres controlled atmosphere glove box, Waters 600 liquid chromatographs (2), Waters detectors (2), Beckman 126 liquid chromatograph and 166 UV detector, Chromatotron rotary chromatograph, Büchi Rotovapor, Varian Cary 3 spectrophotometer with kinetics and Peltier accessories, Eppendorf microcentrifuges (2), water bath, vertical gel electrophoresis systems, power supplies, rotating platform, refrigerator, freezer.

Common rooms: BLSB 204: Beckman J2-21 refrigerated centrifuge, Forma -80°C freezer, Savant Speed-Vacs (2); BLSB 213A: mammalian tissue culture room with Nu-Aire Class II laminar flow hood, Forma CO₂ incubator, Eppendorf high-capacity centrifuge, Olympus inverted fluorescence microscope; BLSB 213: preparation room with Coulter counter, water bath, vertical gel electrophoresis systems, power supplies, rotating platform; BLSB 215: dark room with ultraviolet transilluminators (2), Polaroid CU-5 camera; BLSB 217: 4°C room, with Isco peristaltic pump, Isco fraction collectors (2), and Isco detectors (2); BLSB 218: autoclave and dishwasher; BLSB 235: dark room with Kodak X-Omat film developer.

Animal: N/A.

Computers: BLSB 209/219: IBM x86 1.8 GHz, Dell 1.6 GHz, HP Kayak XM 600 MHz, HP 400 MHz, HP 300 MHz, Compaq 450 MHz (4), Micron 400 MHz, Apple iMac 350 MHz, and IBM 300 MHz (3) desktop computers, Tektronix 740 color laser printer, laser printers (HP, Lexmark, Apple, 6) inkjet printers (Canon, Epson, Lexmark, 6). The computers are networked via university backbone to the KCC Computer Facility, including SGI Indigo2 (2), SGI Challenge-S (3) and HP 735/125 (2) computers, for structural calculations and data base searches.

Offices: BLSB 219A, 90 ft², with Micron 400 MHz PIII computer, Sony Vaio 450 MHz, HP ScanJet IIc digital scanner, and Tektronix 850 color solid ink printer, networked via university backbone; 209B, 250 ft², and 219B, 250 ft², for postdoctoral fellows, graduate students, and project manager.

Major Equipment: BLSB 209B: Jasco J-810 circular dichroism and fluorescence spectrometer with Peltier thermostat, titrator, and stopped-flow kinetics; BLSB 219: Agilent G1600A capillary electrophorometer with diode array detector; BLSB 606: Coulter EPICS flow cytometers (2); BLSB 650: Affinity Sensors IAsys Auto Plus total internal reflectance spectrometer; BLSB 804: Ciphergen SELDI mass spectrometer, ABI 430A peptide synthesizer; BLSB 815: Microarray Facility, with Gene Machines oligonucleotide spotter, Tecan reaction robot, Packard BioScience ScanArray Express fluorescent chip reader, and ABI Prizm 7700 real time PCR fluorescence spectrometer; BLSB 922: Bio-Rad MRC600 confocal fluorescence microscope fitted with Zeiss Axiovert 100 optics; BLSB 1009: Nucleic Acid Facility, with ABI sequencers and primer synthesizers ; BLSB 1026: Molecular Dynamics 445 SI PhosphorImager and Typhoon multimodal imager.

Additional Information: BLSB 302/350: Departmental offices, for purchasing, grant management, and document preparation; Jefferson Alumni Hall: electronics shop, machine shop, media services.

Research Environment: The Kimmel Cancer Center, the Cardeza Foundation for Hematologic Research, the Farber Institute for Neurosciences, and the Jefferson Institute for Molecular Medicine provide a strong nucleus of investigators and facilities for this project. Extramural awards to Thomas Jefferson University for the past year totaled $126 million, of which $83 million were NIH awards.